Web南模生物. Language: English . 中文; 日本語; 한국어; Settings; Exit; Sign in WebAAV is usually considered BSL-1, but may require BSL-97 handling depending on the insert. Storage. Store at -80°C. Thaw just before use and keep on ice. Quality Control. Creative …

AAV pmSyn1-EBFP-Cre in Mouse (AAV Retrograde) - Addgene

WebJAX Example: A good example of this is the Syn1-cre strain that has two genotyping protocols listed. There is a generic cre QPCR protocol that will work for virtually all strains that express cre. There is also a standard PCR protocol that is specific for this particular Syn1-cre. Your choice between these may depend on the breeding scheme that ... WebApr 16, 2024 · In Syn1::Cre brains, we analyzed TdTomato expression with immunofluorescence and noted a marked increase in neurons of Cre(+) animals but not Cre(−) littermates (SI Appendix, Fig. S5C). We then sequenced insertions in Cre(+) and Cre(−) littermates from each line and observed a significant increase in insertion events in … brazil nut kernel

Neuronal - Oxford Academic

WebMar 27, 2024 · It has long been clear that transgenes carrying Cre under the control of a putatively cell type-specific promoter are often expressed in unexpected tissues (Song and Palmiter, 2024; Stifter and Greter, 2024), and here for example our data confirm prior reports of germline activity of Nes Cre and Syn1 Cre drivers (Luo et al., 2024; Betz et al ... WebJul 8, 2024 · Here we compare a widely used “pan-neuronal” mouse Cre driver line, Syn1-cre , with a little-known alternative, Snap25-IRES2-cre . Our results show that the Syn1-cre line … WebSep 10, 2024 · Syn1-Cre mice express Cre recombinase from the rat synapsin-1 promoter in differentiated neuronal cells throughout the brain and spinal cord beginning at E12.5. 26 For the remainder of this study, we compare mixed-sex cohorts of VPS35 flox/flox /Syn1-Cre mice, with the broad neuronal-specific homozygous deletion of VPS35, to their VPS35 flox ... table 웹표준