Phosphopantetheine binding
WebJun 6, 2024 · Unique CFP (cysteine-free protein; 120 aa) has been identified as an extraordinary virulence factor in Beauveria bassiana (Cordycipitaceae), a main source of wide-spectrum fungal insecticides. Its homologs exclusively exist in wide-spectrum insect pathogens of Hypocreales, suggesting their importance for a fungal insect-pathogenic … WebUsing Escherichia coli LipB as a model enzyme, we show here that the octanoyl-transferase mainly recognizes the 4'-phosphopantetheine-tethered acyl-chain of its donor substrate and weakly binds the apo-acyl carrier protein. We demonstrate LipB can accept octanoate from its own ACP and noncognate ACPs, as well as C8-CoA.
Phosphopantetheine binding
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WebView publication. Phosphopantetheine-binding site.: (A) Structural superposition of ATP-bound MsFadD32 and adenylate analog- and phosphopantetheine-bound PA1221 protein. The N- and C-terminal ... Webblocking the malonyl and the CoA/ACP phosphopantetheine-binding sites (10, 13). A wealth of knowledge about the structure, function, and inhibition of the distributed FAS type II system has been gathered over the last decade (14, 15). However, detailed structural studies of the large eukaryotic FAS type I multifunc-
WebSep 4, 2024 · phosphopantetheine binding pocket, away from the solvent channel, forming a hydrogen bond with . Val73, and interacting via water molecules (not shown) with Gly71, Val74 and Asn105 (Figure 2C). WebPhosphopantetheine adenylyltransferase (PPAT) regulates the key penultimate step in the essential coenzyme A (CoA) biosynthetic pathway. PPAT catalyzes the reversible transfer …
WebApr 15, 1999 · The hydrogen bond between the amide of Met74 and the carboxylic oxygen at the elbow of the pantetheine arm stabilizes the bent configuration. In the free protomer, residues 72–74 hinder binding of 4′-phosphopantetheine and would result in steric conflict between Met74 and carboxylic oxygen, thus entailing backbone movement prior to binding. WebPhosphopantetheine tethering is a posttranslational modification that takes place on the active site serine of carrier proteins 138 – ‘acyl carrier proteins ... (for 4′-phosphopantetheine 10). The binding of dephospho-CoA was also shown to be competitive with both ATP and phosphopantetheine, whereas pyrophosphate was competitive with ATP ...
WebJan 10, 2024 · The phosphopantetheine binding site on PCP domain (Ser702) is shown in blue. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article). 4. An asp …
WebJul 1, 2003 · Phosphopantetheine adenylyltransferase (PPAT) regulates the key penultimate step in the essential coenzyme A (CoA) biosynthetic pathway. PPAT catalyzes the reversible transfer of an adenylyl group from Mg2+:ATP to 4′-phosphopantetheine to form 3′-dephospho-CoA (dPCoA) and pyrophosphate. signs of postpartum infectionWebNov 9, 1999 · The first domain (AL) shows 49% similarity to long-chain fatty acid CoA-ligases and contains a putative ATP binding box. The second and fourth domains (ACP) show 45% similarity to acyl carrier proteins, and both contain a putative 4′-phosphopantetheine binding box. The third domain (KS) is 53% similar to β-ketoacyl synthetases. therapielampen testWebNDA 021160/S-015 Page 2 with PhosLo. Progressive hypercalcemia due to overdose of PhosLo may be severe as to require emergency measures. Chronic hypercalcemia may … therapielaserWebSep 29, 2024 · A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and … therapielanfWebAn object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in … therapie landeckWebMar 1, 2024 · It was evident that residues around the phosphopantetheine binding site and the C‐terminal end of helix II of apo‐LipD showed higher intensities from amide exchange experiments, indicating faster exchange (Supporting Information, Fig. S3). The intensities of these residues were suppressed by the attached prosthetic group in holo‐LipD. therapielaser behounekWebThe P5 region is cleaved, resulting in clusters containing only fragments which are attached by the P7 region. This ensures that all copies are sequenced in the same direction. The … therapie lemgo