WebGlycerol present in the loading buffer is used to increase the density of a sample so that it will layer at the bottom of a sample well. So, you need to prepare the gel loading dye and mix... http://www.wendychao.com/science/protocols/loading_buffer/
Loading Dye Science Primer
WebLoading Dye. A colored buffer mixed with a DNA * or other types of samples before loading the samples onto a gel for electrophoresis. The dye contains a relatively high … WebGlycerol Gel-Loading Dye, 5X (Contains 30% Glycerol/Molecular Biol.), Fisher BioReagents. View more versions of this product. Catalog No. BP645-5. This item has … compactpowerinc.com
SDS-PAGE Demystified - PhosphoSolutions
WebWhy this loading dye is superior: 1. The high molecular weight Ficoll-400 stays at the bottom of the well - unlike sucrose or glycerol which diffuse quickly - thus yielding sharper DNA bands. 2. Ficoll can be stored at room temperature, whereas glycerol and sucrose should be refrigerated. WebThe blue protein loading dye contains one vial of blue loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications. 1X Blue Loading Buffer Composition: 62.5 mM Tris-HCl (pH 6.8), 2% (w/v) SDS, 10% glycerol, 0.01% (w/v) bromophenol blue; 30X Reducing Agent: 1.25 M DTT WebApr 19, 2014 · When using small sample volumes, it is advisable to use water to increase the volume and make handling and mixing with loading dye easier. For example, you may mix 2 uL of your sample with 1 uL 6X loading dye and 3 uL water for a final volume of 6 uL. Share Improve this answer Follow answered Apr 19, 2014 at 18:17 jarlemag 3,508 16 26 … compact powerful microwave