Fl83b cells

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WebJan 1, 2024 · FL83B cells were grown in F12K medium supplemented with 10% foetal bovine serum (FBS)(Biological Industries, Haemek, Israel) and 100 mg/L penicillin and streptomycin at 37 °C in a humidified atmosphere of 5% CO2. Cells induced lipid accumulation in hepatocytes using 0.5 mM oleic acid (OA) for 48 h, then 3–100 μM … WebApr 1, 1973 · The FL83B cells are morphologically similar to rat liver parenchymal cells [29], to the granular epithelial cells seen on primary explantation of rat liver [30], and to the secondary liver parenchymal cell line obtained by cloning a suspension of rat liver cells [2]. The question of transformation must be addressed in trying to determine the ...

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WebMay 1, 2024 · FL83B cells were seeded on 6-well plates and incubated with 0.5 mM oleic acid for 48 h. The cells were treated with or without tomatidine (0–10 μM) for 24 h. The plate was washed and the cells fixed with formalin solution before staining with Oil Red O solution to assay oil droplets as described previously [25]. Using an inverted microscope ... WebApr 25, 2024 · Briefly, FL83B cells (~ 18 × 10 6) were collected by centrifugation and sonicated in cold diluted assay buffer. The supernatant was removed, and cells were re-suspended in cold diluted assay buffer. BHB standard wells and sample wells were prepared as per the protocol. The reaction was initiated by adding developer solution. fishersville gun show 2021

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WebThe FL83B cells were treated with insulin for 5 or 10 min. GRK2 was labeled with anti-GRK2 antibody and Cy3-conjugated secondary antibody and the cells were observed under the microscope. Insulin treatment increased the translocation of GRK2 from cytosol to the plasma membrane ( Fig. 1A ). WebFeb 6, 2013 · FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent d-glucose derivative, was performed, … WebMay 19, 2024 · FL83B cells were seeded in six-well plates at 4x10 5 cells per well with high-glucose DMEM containing 10 vol% FBS, penicillin-streptavidin-amphotericin B (Gibco … can animals see infrared light

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WebDec 9, 2024 · FL83B cells were harvested at the exponential growth phase, prepared into a suspension of approximately 1 × 10 6 cells/mL, and 600 μL of this suspension was added to the lower chamber. FL83B cells were stimulated with 40 ng/mL TNF-α for 2 h in model group (+ TNF-α group). FL83B cells were stimulated with an equal volume of PBS for 2 … WebFAM83A-H are newly identified oncogenes characterised by a conserved DUF1669 domain. FAM83B can substitute for Ras to promote malignant transformation. Ablation of … fishersville fasmaWebJan 12, 2024 · FL83B cells were co-transfected with pDR1 and pRL-TK (Promega, Seattle, WA, USA) containing CMV promoter-controlled Renilla luciferase gene using fuGENE HD (Promega, Seattle, WA, USA), and incubated with serum free media for 24 hr. Then the cells were treated with FFA and AM as described above, and, another 24 hr later, the … fishersville graphic design

"WebS-Allyl cysteine attenuates free fatty acid-induced lipogenesis in human HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway ☆ Author links open overlay panel Yong Pil Hwang a b 1 , Hyung Gyun Kim a 1 , Jae Ho Choi a , Minh Truong Do a , Young Chul Chung c , Tae Cheon Jeong d , Hye Gwang Jeong a " - Fl83b cells

Fl83b cells

Fucoxanthin attenuates fatty acid-induced lipid accumulation in FL83B …

WebAug 1, 2007 · The present study was designed to test the hypothesis that GRK2 negatively regulates IR signaling. FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of ... WebFL83B cells were obtained from the Taiwan Bioresource Collection and Research Center (BCRC number: 60325) and cultured in F12K medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified atmosphere with 5% CO 2. To make the stock medium containing 4 mM OLA, sodium oleate was conjugated …

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WebJul 13, 2007 · FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of GRK2 was determined using immunofluoresecence and … WebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot …

WebMay 25, 2011 · Finally, the possible mechanism of DPS promoting insulin sensitivity in TNF-α-induced FL83B cells was investigated in this study. DPS may attenuate c-Jun N … Web(F) Lipid content analysis of FL83B cells treated with 400 mM OA and different CLH concentrations by using Nile red stain. The data are given as mean ± SEM (n ¼ 6).

WebMay 21, 2024 · Abstract. FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its … WebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot …

WebJul 13, 2024 · FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non-targeting agRNA (control) and Tcf4 or Mafk sgRNA.

WebApr 1, 1973 · FL83B is a normal cell line of hepatocytes from a C57BL/6J mouse of 15–17 days of gestation (Breslow et al., 1973). FL83B required F-12 K medium with 10% FBS. … fishersville fergusonWebJul 13, 2024 · Characteristics. tissue/cell type: Liver. condition: Healthy. treatment: none. Treatment protocol. FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non … fishersville hospitalWebNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum (FBS), 100 mg/mL penicillin and 0.25 mg/mL … fishersville gun showWebDec 29, 2015 · FL83B cell line represent normal hepatocytes whereas hHSC cell line has the angiotensin 1 receptors which is the main target for losartan receptors. Confocal … fishersville gymWebCells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and … fishersville heritage antique marketWebDec 1, 2024 · Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF-α-treated FL83B cells. Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at … fishersville internal medicine vaWebOriginal magnification: 100×. (C) FL83B cells were transfected with HA-Daxx and then treated with or without TGF-β for 24 h. Western blotting analysis of Daxx, E-cadherin and Vimentin expression. Relative expression was normalized to β-actin expression as a reference. The graph shows quantitative densitometry from Western blotting. fishersville internal medicine